Nutrient Broth

Microbiology Culture Media

Specifications

• Product Name: Nutrient Broth
• Type: General-purpose liquid culture media
• Form: Dehydrated powder
• Color: Light yellow after preparation
• Composition (approx.):
  • Peptone: 5 g/L
  • Beef Extract: 3 g/L
  • Sodium Chloride: 5 g/L
  • Distilled Water: 1 Liter
  • Final pH: 7.4 ± 0.2 at 25°C
• Preparation: Dissolve 13 g in 1 liter distilled water, mix well until completely dissolved, sterilize by autoclaving at 121°C for 15 minutes
• Packaging: 500 gram
• Origin: Imported

Applications

• Used for cultivation of a wide variety of non-fastidious microorganisms
• Provides nutrients for bacterial growth in liquid form
• Common in teaching microbiology in schools and universities
• Suitable for sub-culturing bacteria before further testing
• Often used as a base medium for enrichment

Safety & Handling

• Store in cool, dry conditions away from direct sunlight
• Avoid inhalation of powder, wear gloves and protective clothing
• Dispose of broth cultures as per laboratory biosafety rules

Sterilize media and glassware before disposal